three-dimensional model of salmonella s needle

2012

2016-1-20Schraidt O Marlovits T C Three-dimensional model of Salmonella's needle com-plex at subnanometer resolution Science331 1192-1195 (2011) PDB ID: 2y9j 2y9k Bernal-Bayard J Ramos-Morales F Salmonellatype III secretion effector SlrP is an E3 ubiquitin ligase for mammalian thioredoxin J Biol Chem 284 27587-27595 (2009)

Salmonella T3SSs: successful mission of the secret(ion

2013-2-1Bacteria of the genus Salmonella express nanosyringe-like organelles called type three secretion systems (T3SSs) These systems promote the secretion of bacterial compounds and their translocation into host cells Pathogenic Salmonella use two distinct T3SSs with specialized functions having the purpose to modify the biology of the host organism and to ensure a successful infection

Three

Three-Dimensional Model of Salmonella's Needle Complex at Subnanometer Resolution Author: Schraidt Oliver Marlovits Thomas C Source: Science 2011 v 331 no 6021 pp 1192-1195 ISSN: 0036-8075 Subject: Gram-negative bacteria Salmonella Typhimurium cryo-electron microscopy models type III secretion system virulence Abstract:

A' B' FliF

2016-2-18PDBTitle: three-dimensional model of salmonella's needle complex at2 subnanometer resolution 3 c2qsfX_ Alignment 45 0 23 PDB header:dna binding protein Chain: X: PDB Molecule:uv excision repair protein rad23 PDBTitle: crystal structure of the rad4-rad23 complex 4 c3uz0A_ Alignment 40 5 20 PDB header:transport protein

A' B' FliF

2016-2-18PDBTitle: three-dimensional model of salmonella's needle complex at2 subnanometer resolution 3 c2qsfX_ Alignment 45 0 23 PDB header:dna binding protein Chain: X: PDB Molecule:uv excision repair protein rad23 PDBTitle: crystal structure of the rad4-rad23 complex 4 c3uz0A_ Alignment 40 5 20 PDB header:transport protein

2Y9K

cansSAR 3D Structure of 2Y9K | THREE-DIMENSIONAL MODEL OF SALMONELLA'S NEEDLE COMPLEX AT SUBNANOMETER RESOLUTION | 2Y9K_A | Type 3 secretion system secretin - Also known as SCTC_SALTY sctC invG Component of the type III secretion system (T3SS) also called injectisome which is used to inject bacterial effector proteins into eukaryotic host cells

Visualization of the type III secretion sorting platform

Many infectious bacteria such as Shigella and Salmonella use type III secretion machines also called injectisomes to transfer virulence proteins into eukaryotic host cells A cytoplasmic sorting platform is required for effector selection and assembly of the needle but has not been visualized in any bacteria We combine advanced imaging and genetic techniques to visualize the frozen-hydrated

The Three

Three-dimensional reconstructions from electron cryomicrographs of the rotor of the flagellar motor reveal that the symmetry of individual M rings varies from 24-fold to 26-fold while that of the C rings containing the two motor/switch proteins FliM and FliN varies from 32-fold to 36-fold with no apparent correlation between the symmetries of the two rings

NMR Characterization of the Interaction of the

2019-12-12Salmonella and Shigella bacteria require the type III secretion system (T3SS) to inject virulence proteins into their hosts and initiate infections The tip proteins SipD and IpaD are critical components of the Salmonella and Shigella T3SS respectively Recently SipD and IpaD have been shown to interact with bile salts which are enriched in the intestines and are hypothesized to act as

Analysis of Interactions of Salmonella Type Three

Three-dimensional growth of extravillous cytotrophoblasts promotes differentiation and invasion (2006) Three-dimensional organotypic models of human colonic epithelium to study the early stages of enteric salmonellosis (2001) Three-dimensional tissue assemblies: novel models for the study of Salmonella enterica serovar Typhimurium pathogenesis

Building a secreting nanomachine: a structural

2014-4-1(b) A cartoon representation of the Salmonella enterica needle with N-terminal out subunit built using Rosetta by Loquet et al (PDB-2lpz [16 ••]) (c) Trivial remodeling of the flexible N-terminus of the Loquet et al [16 ••] subunit position demonstrates a good fit of this independently derived needle model to the Fuji et al EM map

Type three secretion system in Salmonella

2020-2-28Type Three Secretion Systems (T3SS) are nanomachine complexes which display the ability to inject effector proteins directly into host cells This skill allows for gram-negative bacteria to modulate several host cell responses such as cytoskeleton rearrangement signal transduction and cytokine production which in turn increase the pathogenicity of these bacteria The Salmonella

Three

2011-3-1Type III secretion systems (T3SSs) are essential virulence factors used by many Gram-negative bacteria to inject proteins that make eukaryotic host cells accessible to invasion The T3SS core structure the needle complex (NC) is a ~3 5 megadalton-sized oligomeric membrane-embedded complex Analyzing cryo-electron microscopy images of top views of NCs or NC substructures from Salmonella

Salmonella T3SSs: successful mission of the secret(ion

2013-2-1Bacteria of the genus Salmonella express nanosyringe-like organelles called type three secretion systems (T3SSs) These systems promote the secretion of bacterial compounds and their translocation into host cells Pathogenic Salmonella use two distinct T3SSs with specialized functions having the purpose to modify the biology of the host organism and to ensure a successful infection

NMR Characterization of the Interaction of the

2019-12-12Salmonella and Shigella bacteria require the type III secretion system (T3SS) to inject virulence proteins into their hosts and initiate infections The tip proteins SipD and IpaD are critical components of the Salmonella and Shigella T3SS respectively Recently SipD and IpaD have been shown to interact with bile salts which are enriched in the intestines and are hypothesized to act as

SipD and IpaD induce a cross

2020-5-28The structural proteins composing the T3SS are common to all virulent Salmonella and Shigella spp particularly the needle-tip proteins SipD (Salmonella) and IpaD (Shigella) We investigated the immunogenicity and protective efficacy of SipD and IpaD administered by intranasal and intragastric routes in a mouse model of Salmonella enterica

Building a secreting nanomachine: a structural

2014-4-1(b) A cartoon representation of the Salmonella enterica needle with N-terminal out subunit built using Rosetta by Loquet et al (PDB-2lpz [16 ••]) (c) Trivial remodeling of the flexible N-terminus of the Loquet et al [16 ••] subunit position demonstrates a good fit of this independently derived needle model to the Fuji et al EM map

RCSB PDB

As a member of the wwPDB the RCSB PDB curates and annotates PDB data according to agreed upon standards The RCSB PDB also provides a variety of tools and resources Users can perform simple and advanced searches based on annotations relating to sequence structure and function These molecules are visualized downloaded and analyzed by users who range from students to specialized scientists

Atomic model of the type III secretion system needle

Pathogenic bacteria using a type III secretion system (T3SS) 1 2 to manipulate host cells cause many different infections including Shigella dysentery typhoid fever enterohaemorrhagic colitis and bubonic plague An essential part of the T3SS is a hollow needle-like protein filament through which effector proteins are injected into eukaryotic host cells 3–6

Crystal Structure of PrgI

2011-8-4Three dimensional model of the T3SS needle tip The cryo-EM map of isolated needles from S flexneri which is similar to the map obtained from isolated S typhimurium needle together with the X-ray crystal structure of a needle protomer mutant can be used to build a composite 3-dimensional model

Molecular ruler determines needle length for the

S enterica encodes two injectisomes: the Salmonella pathogenicity island 1 (Spi-1) and Salmonella pathogenicity island 2 (Spi-2) injectisomes which are required to cause gasteroenteritis in humans The Spi-1 needle is composed of PrgI subunits that polymerize to a length of ∼25 nm before undergoing the secretion specificity switch

Visualization of the type III secretion sorting platform

Many infectious bacteria such as Shigella and Salmonella use type III secretion machines also called injectisomes to transfer virulence proteins into eukaryotic host cells A cytoplasmic sorting platform is required for effector selection and assembly of the needle but has not been visualized in any bacteria We combine advanced imaging and genetic techniques to visualize the frozen-hydrated

2012

2016-1-20Schraidt O Marlovits T C Three-dimensional model of Salmonella's needle com-plex at subnanometer resolution Science331 1192-1195 (2011) PDB ID: 2y9j 2y9k Bernal-Bayard J Ramos-Morales F Salmonellatype III secretion effector SlrP is an E3 ubiquitin ligase for mammalian thioredoxin J Biol Chem 284 27587-27595 (2009)

Three dimensional structure of the injectisome

2020-7-17Needle complex isolated from the membrane of Salmonella typhimurium Resolution 11 7 Contour Level 0 038 Attribution PubMed ID 21 385 715 Digital Object Identifier (DOI) 10 1126/science 1199358 Author(s) Schraidt O Marlovits TC Citation Title Three-dimensional model of Salmonella's needle complex at subnanometer resolution

2Y9K

cansSAR 3D Structure of 2Y9K | THREE-DIMENSIONAL MODEL OF SALMONELLA'S NEEDLE COMPLEX AT SUBNANOMETER RESOLUTION | 2Y9K_A | Type 3 secretion system secretin - Also known as SCTC_SALTY sctC invG Component of the type III secretion system (T3SS) also called injectisome which is used to inject bacterial effector proteins into eukaryotic host cells

2Y9J: Three

2Y9J: Three-dimensional Model of Salmonella's Needle Complex at Subnanometer Resolution: PDB ID: 2Y9J Download: MMDB ID: 89332: PDB Deposition Date: Three-dimensional model of salmonella's needle complex at subnanometer resolution Schraidt O Marlovits TC Science (2011) 331 p 1192