protein immobilization strategies for protein biochips

Perspective: A toolbox for protein structure

2019-3-20Revealing the structure of complex biological macromolecules such as proteins is an essential step for understanding the chemical mechanisms that determine the diversity of their functions Synchrotron based X-ray crystallography and cryo-electron microscopy have made major contributions in determining thousands of protein structures even from micro-sized crystals

His

2010-10-28protein microarrays a subset of the field of protein biochips (2) broadly defined as proteins immobilized on flat surfaces The report by Grunwald et al (3) provides a significant advance in the spatially defined immobilization of proteins on surfaces It accomplishes this task using a novel para-digm for photochemical activation: Bind-

Immobilization of proteins to biacore sensor chips

Abstract The immobilization of proteins to surfaces is an active area of research due to strong interest in protein-based sensors Here we describe a novel method for immobilizing ligand proteins onto Biacore sensor chips using the transpeptidase activity of Staphylococcus aureus sortase A (SrtA) This method provides a robust and gentle approach for the site-directed covalent coupling of

Chemical Strategies for Generating Protein Biochips

Chemical Strategies for Generating Protein Biochips Pascal Jonkheijm Dr Department of Chemical Biology Max Planck Institute of Molecular Physiology and Faculty of Chemistry Chemical Biology Technical University of Dortmund Otto Hahn Strasse 11

Protein chips: from concept to practice: Trends in

Protein chips have emerged as an exciting technology for the broad characterization of the activities and inter-actions of proteins Like the gene-chip technology that motivated this development protein chips will prove to be essential to researchers in biology and to commercial programs in

Protein immobilization strategies for protein biochips

T1 - Protein immobilization strategies for protein biochips AU - Rusmini F AU - Rusmini Federica AU - Zhong Zhiyuan AU - Feijen Jan N1 - review paper PY - 2007 Y1 - 2007 N2 - In the past few years protein biochips have emerged as promising proteomic and diagnostic tools for obtaining information about protein functions and interactions

Immobilization of His

The immobilization capacity of our method was evaluated using His-tagged GFP (Green Fluorescent Protein) as a model protein The average immobilization density on modified glass was about 32 ng/mm2 Our method is suitable for use on a variety of solid surfaces including glassy carbon silicon wafers polycarbonate and beaten gold

Recent advances in covalent site

2016-9-12Introduction Protein immobilization plays an important role in the fields of life science and medicine It forms the basis for many bio-based applications involving protein–protein or protein–ligand interactions such as biosensors biomedical implants recyclable biocatalysts and protein arrays for drug screening 1 For these devices to perform in an optimal manner a high level of

Protein immobilization strategies for protein biochips

In this review current protein immobilization strategies including physical covalent and bioaffinity immobilization for the fabrication of protein biochips are described Particular consideration has been given to oriented immobilization also referred to as site-specific immobilization which is believed will improve homogeneous surface

Protein and Chemical Microarrays

Similar strategies can in principle be applied to protein or chemical microarrays as well IMMOBILIZATION VIA COVALENT ATTACHMENT Although nonspecific physical adsorption has been used successfully for generating a microarray of macromolecules this approach is less useful for the preparation of small molecule or small peptide microarrays

The Research Progress of Antibody Immobilization

Soellner M B Dickson K A Nilsson B L et al Site-specific protein immobilization by staudinger ligation Journal of the American Chemical Society 2003 125(39):11790-11791 doi: 10 1021/ja036712h pmid: 14505380 [46] Kohn M Immobilization strategies

Recent advances in covalent site

2016-9-12Introduction Protein immobilization plays an important role in the fields of life science and medicine It forms the basis for many bio-based applications involving protein–protein or protein–ligand interactions such as biosensors biomedical implants recyclable biocatalysts and protein arrays for drug screening 1 For these devices to perform in an optimal manner a high level of

Orientation and characterization of immobilized

2019-1-29Immunodiagnostics protein biochips and biosensors employed for antigen detection and quantification from bio-logical samples often employ recognition proteins such as antibodies 1–4 Assay sensitivity is dependent on immobiliza-tion of the capture antibodies onto a solid support with a suf-

Temperature Characteristic of Immobilization Proteins

[8] Federica Rusmini Zhiyuan Zhong Jan Feijen Protein Immobilization Strategies for Protein Biochips [J] Biomacromolecules 8(2007) 1775-1789 DOI: 10 1021/bm061197b [9] Piletsky S Pletska E Bossi A et al Surface functionalization of porous polypropylene membranes with polyaniline for protein immobilization [J]Biotechnol

Hydrogel based protein biochip for parallel detection

2019-12-2The Systemic Inflammatory Response Syndrome (SIRS) a sepsis related inflammatory state is a self-defense mechanism against specific and nonspecific stimuli The six most extensively studied inflammatory biomarkers for the clinical diagnosis of SIRS are interleukin 4 (hIL-4) interleukin 6 (hIL-6) interleukin 10 (hIL-10) tumor necrosis factor alpha (hTNF-α) interferon gamma (hIFN-γ) and

Oriented immobilization of proteins on solid supports

Immobilization of proteins on a solid support is critical with respect to the fabrication and performance of biosensors and biochips Protein attachment with a preferable orientation can effectively avoid its denaturation and keeps its active sites fully exposed to solution thus maximally preserving the bioaffinity or bioactivity This review (with 140 refs ) summarises the recent advances in

Towards quantitative assays with peptide chips: a

Recently several groups have developed peptide and protein arrays and applied them to a range of applications Here three surface-engineering strategies for microarray design are described that promise to widen the application of microarrays by permitting the evaluation of hits in a chip-based format

The Research Progress of Antibody Immobilization

Soellner M B Dickson K A Nilsson B L et al Site-specific protein immobilization by staudinger ligation Journal of the American Chemical Society 2003 125(39):11790-11791 doi: 10 1021/ja036712h pmid: 14505380 [46] Kohn M Immobilization strategies

Chemical Strategies for Generating Protein Biochips

Chemical Strategies for Generating Protein Biochips Pascal Jonkheijm Dr Department of Chemical Biology Max Planck Institute of Molecular Physiology and Faculty of Chemistry Chemical Biology Technical University of Dortmund Otto Hahn Strasse 11

Characterization of Recombinant His

1 Introduction Protein immobilization on solid supports has become an essential process to develop biomaterials including biosensors and to study protein-protein interactions and analyte-protein interactions [1 2] The main challenges in protein immobilization are maintaining the protein functionality and controlling the orientation to ensure equal access of the analyte to the active sites

Recent advances in covalent site

2016-9-12Introduction Protein immobilization plays an important role in the fields of life science and medicine It forms the basis for many bio-based applications involving protein–protein or protein–ligand interactions such as biosensors biomedical implants recyclable biocatalysts and protein arrays for drug screening 1 For these devices to perform in an optimal manner a high level of

Applications of Protein Biochips in Biomedical and

Progress in the development of protein‐immobilization strategies and methods has made protein biochips increasingly accessible The integration of these assay and analysis platforms into biomedical and biotechnological research has substantially expanded the repertoire of methods available for proteomics and biomarker research and for drug development This Minireview highlights selected

Specific and Reversible Immobilization of Proteins

2014-1-31Rusmini F Zhong Z Feijen J (2007) Protein immobilization strategies for protein biochips Biomacromolecules 8: 1775–1789 View Article Google Scholar 8 Bilitewski U (2006) Protein-sensing assay formats and devices Anal Chim Acta 568: 232–247 View Article

Protein Immobilization Strategies for Protein Biochips_

2007-4-20In this review current protein immobilization strategies including physical covalent and bioaffinity immobilization for the fabrication of protein biochips are described Particular consideration has been given to oriented immobilization also referred to as site-specific immobilization which is believed will improve homogeneous surface covering and accessibility of the active site

Molecules

Protein microarrays represent important tools for biomedical analysis We have recently described the use of the biarsenical-tetracysteine (TC) tag for the preparation of protein microarrays The unique feature of this tag enables the site-specific immobilization of TC-containing proteins on biarsenical-modified surfaces resulting in a fluorescence enhancement that allows the direct

Dendrimeric coating of glass slides for sensitive DNA

Received April 1 2003 Revised and Accepted June 3 2003 INTRODUCTION DNA microarrays are produced by in situ synthesis of oligonucleotides or by mechanical deposition of pre‐fabricated nucleotides onto chemically reactive support by means of ink jetting or spotting robot devices (2 3) The activated glass plates (e g microscope slides) are readily available easy to handle and commonly

Protein immobilization strategies for protein biochips

Although a large number of review papers on protein biochips have been published in recent years few have focused on protein immobilization technology In this review current protein immobilization strategies including physical covalent and bioaffinity immobilization for the fabrication of protein biochips are described

efficient method for enzyme immobilization

Introduction Protein immobilization is a decisive step in the surface-based analysis consisting of protein–protein (or ligand) interactions In the last years many research lines have been devoted to establish stable and strong protein attachment onto different kinds of surfaces to develop microarray-based proteome analysis (MacBeath and Schreiber 2000 Kwon et al 2007 Tao and Zhu

Covalent and Selective Immobilization of Fusion

2003-6-10Protein Immobilization Strategies for Protein Biochips Biomacromolecules 2007 8 (6) 1775-1789 DOI: 10 1021/bm061197b Ranganath Parthasarathy Shyamsundar Subramanian and Eric T Boder Sortase A as a Novel Molecular "Stapler" for Sequence-Specific Protein Conjugation